Epitalon and melatonin for anti-ageing care!

Dear readers,

Firstly thank you for your registration, and thank you for your interest in your own well-being and your health and longevity.

Here is some essential information to fight against aging:

Epitalon stimulates and re-establish the production of melatonin, an essential factor to stay in good health !

Here is a short selection of studies already published :

This one concerns epitalon and melatonin that are able to correct reproduction cycles in rats :
– PMID: 23237594 : Melatonin and pineal gland peptides are able to correct the impairment of reproductive cycles in rats.
Arutjunyan A, Kozina L, Milyutina Y, Korenevsky A, Stepanov M, Arutyunov V.
Curring Aging Sci. 2012 Dec;5(3):178-85.

This one concerns molecular cellular mechanisms of melatonin synthesis by epitalon :
– PMID: 22816096 : Molecular cellular mechanisms of peptide regulation of melatonin synthesis in pinealocyte culture.
Khavinson VKh, Linkova NS, Kvetnoy IM, Kvetnaia TV, Polyakova VO, Korf HW.
Bull Exp Biol Med. 2012 Jun;153(2):255-8. English, Russian.

This one concerns the regulatory effect of epitalon on melatonin and cortisol production :
– PMID: 11550036 : Regulatory effect of Epithalon on production of melatonin and cortisol in old monkeys.
Goncharova ND, Khavinson BK, Lapin BA.
Bull Exp Biol Med. 2001 Apr;131(4):394-6.

This one concerns the effects of epitalon and melatonin on ageing of thyroid and hypophysis :
– PMID: 20405731 : Effect of preparations melatonin and epitalon on the age-related dynamics of thyrotrophic activity of the hypophysis and thyroid gland function in different light regimes.
Vinogradova IA.
Adv Gerontol. 2009;22(4):631-8. Russian.

This one concerns the effect of epitalon on melatonin production by the pineal gland :
– PMID: 12809170 : Effect of a synthetic pineal tetrapeptide (Ala-Glu-Asp-GLy) on melatonin secretion by the pineal gland of young and old rats.
Djeridane Y, Khavinson VKh, Anisimov VN, Touitou Y.
J Endocrinol Invest. 2003 Mar;26(3):211-5.

This one concerns epitalon correcting neuroendocrine regulations in old monkeys :
– PMID: 11524632 : Synthetic tetrapeptide epitalon restores disturbed neuroendocrine regulation in senescent monkeys.
Khavinson V, Goncharova N, Lapin B.
Neuro Endocrinol Lett. 2001 Aug;22(4):251-4.

This one concerns the influence of epitalon and melatonin on the number of leukocytes :
– PMID: 19432172 : The influence of melatonin and epithalon on blood leukocyte count and leukocyte alkaline phosphatase in rats under different lighting conditions during ontogenesis.
Uzenbaeva LB, Vinogradova IA, Golubeva AG, Niuppieva MG, Iliukha VA.
Adv Gerontol. 2008;21(3):394-401. Russian.

 

[1] Melatonin and Epitalon, studies on PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/?term=melatonin+epitalon

BPC 157 as an effective therapy for muscle crush injury

This trial shows that BPC157 accelerates muscle healing and to restore full muscle function!

Surgery Today Journal
August 2008, Volume 38, Issue 8, pp 716-725

Gastric pentadecapeptide BPC 157 as an effective therapy for muscle crush injury in the rat
Purpose
Stable gastric pentadecapeptide BPC 157 accelerates the healing of a transected Achilles tendon and a transected quadriceps muscle. It may also be of clinical relevance as a systemic and local peptide treatment for crush injury of a major muscle, such as gastrocnemius muscle complex. BPC 157 is effective without a carrier, and it is presently undergoing trials for inflammatory bowel disease, and no toxicity has so far been reported.

Methods
In crushed rats (force delivered 0.727 Ns/cm2), BPC 157 was applied either intraperitoneally or locally, as a thin cream layer, immediately after injury (sacrifice at 2 h), and once a day for 14 days.

Results
BPC 157 improved muscle healing, macroscopically (less hematoma and edema, no post-injury leg contracture), microscopically, functionally, and also based on enzyme activity (creatine kinase, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase).

 

Conclusion
BPC 157, at all investigated intervals, given locally or intraperitoneally, accelerated post-injury muscle healing and also helped to restore the full function.

 

Key words
BPC 157 Crush injury Peptide therapy Muscle regeneration Rat

Source for this article: http://link.springer.com/article/10.1007%2Fs00595-007-3706-2

 

Useful References:

  • 1. Sikiric P, Petek M, Rucman R, Seiwerth S, Grabarevic Z, Rotkvic I, et al. A new gastric peptide BPC. An overview of stomach — organoprotection hypothesis and beneficial effect of BPC. J Physiol (Paris) 1993;87:313–327.
  • 2. Sikiric P, Seiwerth S, Brcic L, Blagaic AB, Zoricic I, Sever M, et al. Stable gastric pentadecapeptide BPC 157 in trials for inflammatory bowel disease (PL-10, PLD-116, PL 14736, Pliva, Croatia). Full and distended stomach, and vascular response. Inflammopharmacology 2006;14:214–221.
  • 4. Jelovac N, Sikiric P, Rucman R, Petek M, Marovic A, Perovic D, et al. Pentadecapeptide BPC 157 attenuates disturbances induced by neuroleptics: the effect on catalepsy and gastric ulcers in mice and rats. Eur J Pharmacol 1999;379:19–31.
  • 5. Sikiric P, Seiwerth S, Grabarevic Z, Rucman R, Petek M, Jagic V, et al. Beneficial effect of a novel pentadecapeptide BPC 157 on gastric lesions induced by restraint stress, ethanol, indomethacin, and capsaicin neurotoxicity. Dig Dis Sci 1996;41:1604–1614.
  • 6. Sikiric P, Seiwerth S, Grabarevic Z, Rucman R, Petek M, Jagic V, et al. The influence of a novel pentadecapeptide, BPC 157, on N(G)-nitro-L-arginine methylester and L-arginine effects on stomach mucosa integrity and blood pressure. Eur J Pharmacol 1997;332:23–33.
  • 7. Sikiric P, Seiwerth S, Mise S, Staresinic M, Bedekovic V, Zarkovic N, et al. Corticosteroid-impairment of healing and gastric pentadecapeptide BPC-157 creams in burned mice. Burns 2003;29:323–334.
  • 8. Veljaca M, Chan K, Guglietta A. Digestion of h-EGF, h-TGF alpha, and BPC-15 in human gastric juice. Gastroenterology 1995;108:761.
  • 12. Staresinic M, Petrovic I, Novinscak T, Jukic I, Pevec D, Suknaic S, et al. Effective therapy of transected quadriceps muscle in rat: Gastric pentadecapeptide BPC 157. J Orthop Res 2006;24:1109–1117.
  • 14. Krivic A, Anic T, Seiwerth S, Huljev D, Sikiric P. Achilles detachment in rat and stable gastric pentadecapeptide BPC 157: promoted tendon-to-bone healing and opposed corticosteroid aggravation. J Orthop Res 2006;24:982–989.
  • 15. Staresinic M, Sebecic B, Patrlj L, Jadrijevic S, Suknaic S, Perovic D, et al. Gastric pentadecapeptide BPC 157 accelerates healing of transected rat Achilles tendon and in vitro stimulates tendocytes growth. J Orthop Res 2003;21:976–983.
  • 16. Sebecic B, Nikolic V, Sikiric P, Seiwerth S, Sosa T, Patrlj L, et al. Osteogenic effect of a gastric pentadecapeptide, BPC-157, on the healing of segmental bone defect in rabbits: a comparison with bone marrow and autologous cortical bone implantation. Bone 1999;24:195–202.
  • 17. Bilic M, Bumber Z, Blagaic AB, Batelja L, Seiwerth S, Sikiric P. The stable gastric pentadecapeptide BPC 157, given locally, improves CO2 laser healing in mice. Burns 2005;31:310–315.
  • 18. ikus D, Sikiric P, Seiwerth S, Petricevic A, Aralica G, Druzijanic N, et al. Pentadecapeptide BPC 157 cream improves burn-wound healing and attenuates burn-gastric lesions in mice. Burns 2001;127:817–827
  • 19. Seiwerth S, Sikiric P, Grabarevic Z, Zoricic I, Hanzevacki M, Ljubanovic D, et al. BPC 157’s effect on healing. J Physiol (Paris) 1997;91:173–178.
  • 21. Xue XC, Wu YJ, Gao MT. Study of the protective effects of pentadecapeptide BPC 157 on wounds in small type pigs. Chin New Drugs J 2004;12:602–604.
  • 23. Gjurasin M, Miklic P, Buljat G, Perovic D, Jukic I, Sikiric P, et al. Selective healing effect of the gastric pentadecapeptide BPC 157 on injured part of the nerve in subtotal sciatic nerve transection. Dig Dis Sci 2003;48:1878.
  • 24. Vuksic T, Zoricic I, Brcic L, Sever M, Klicek R, Radic B, et al. Stable gastric pentadecapeptide BPC 157 in trials for inflammatory bowel disease (PL-10, PLD-116, Pliva, Croatia) heals ileoileal-anastomosis in rat. Surg Today 2007;37:768–777.
  • 34. Lovric-Bencic M, Sikiric P, Hanzevacki JS, Seiwerth S, Rogic D, Kusec V, et al. Doxorubicine-congestive heart failure-increased big endothelin-1 plasma concentration: reversal by amlodipine, losartan, and gastric pentadecapeptide BPC 157 in rat and mouse. J Pharmacol Sci 2004;95:19–26.
  • 35. Prkacin I, Separovic J, Aralica G, Perovic D, Gjurasin M, Lovric-Bencic M, et al. Portal hypertension and liver lesions in chronically alcohol drinking rats prevented and reversed by stable gastric pentadecapeptide BPC 157 (PL-10, PLD-116), and propranolol, but not ranitidine. J Physiol (Paris) 2001;95:315–324.
  • 37. Sikiric P, Separovic J, Anic T, Buljat G, Mikus D, Seiwerth S, et al. The effect of pentadecapeptide BPC 157, H2 blockers and sucralfate on new vessels and new granulation tissue formation. J Physiol (Paris) 1999;93:479–485.
  • 38. Veljaca M, Lesch CA, Sanchez B, Low J, Guglietta A. Protection of BPC-15 on TNBS-induced colitis in rats: possible mechanisms of action. Gastroenterology 1995;108:936.
  • 39. Veljaca M, Lesch CA, Pllana R, Sanchez B, Chan K, Guglietta A. BPC-15 reduces trinitrobenzene sulfonic acid-induced colonic damage in rats. J Pharmacol Exp Ther 1994;272:417–422.

It repairs and reverses the aging of our DNA, it is proven!

Hello everyone,

More and more companies and scientists are investing their time and money in research against aging. For 10 years the tracks have been widening and the results are accumulating.

Yes, we can already act, and indeed for a few years already, by adapting our way of life and nourishment, but above all by optimizing the functioning of our cells because they are indeed the basis of our physical being.

You know of course that I have taken the bull by the horns since 2012, after a diagnosis of prostate tumor. And that I had the immense chance to try epitalon, which straight from the first days gave me fantastic results, because my very troublesome symptoms disappeared almost as if by a miracle!

This high-dose epitalon then deeply rejuvenated me. (You can read my testimony here)

Combating aging is therefore possible. Rejuvenating is a real reality, and I invite you to try to have proof for yourself.

Then in 2015 Biotrends added another layer, making available to individuals its second anti-aging product after epitalon!

This molecule that brings pure energy to all our cells, and that does even more than that according to scientists!

The french magazine Health Science and Conscience also speaks of it in its latest June issue.

It mentions that scientists have identified a cellular mechanism that reverses the aging of DNA!

It is thus possible to activate the DNA repair process, but also to cancel out the existing effects.

It was in 2013 that Professor Sinclair and his team discovered that the cells of young mice contain more oxidized Nicotinamide Adenine Dinucleotide than those of older mice. And by injecting them with this molecule, they showed all the signs of youth, proving that they had rejuvenated.

As you had guessed I am sure, this is NAD+, a form of pure energy that allows cells to start working instantly to repair and multiply. It is a shortcut to reinvigorate our aged cells which are tired and have problems to produce their operating energy because of the rarefaction of their mitochondria.

Since then, scientific studies have continued, and Professor Sinclair has understood in detail the other mechanism of rejuvenation of NAD+, which is the protection of DNA against the damage caused by aging and radiation.

Our cells gradually lose their ability to repair the errors of mutation and degradation of our DNA during their divisions.

But when there is a lot of NAD+ present in the cell, it prevents this damage on the DNA!

This mechanism of rejuvenation is already exploited by many companies that market a precursor of NAD+, ie a molecule that can potentially transform into NAD+.

But this precursor, although cheaper, has only a very weak action against aging compared to true NAD+.

The NAD+ manufactured by Biotrends is the real and only product that can really give you an awesome rejuvenation shot. Of course it’s because it is more complicated and expensive to manufacture that large groups give up on it.

Their cheap precursor called NMN will not help you, you will see it for yourself if you try it.

On the other hand, pure NAD+ that will be directly usable by your cells will give you a surprising effect, as was the case for me.

I actually felt a growing strength in me, only a few tens of minutes after taking the equivalent of 300mg of NAD+.

At the time it was small pills of only 30 mg each, while now they are 100 mg capsules or 60 grams powder for even more spectacular effects, and at lower cost.

 

Professor Sinclair’s team now works with NASA to protect astronauts from DNA degradation caused by the powerful cosmic radiation in space. Especially in preparation for trips to Mars.

NAD+ will therefore help people who often fly, or undergo x-rays, but also sportsmen for whom it is a source of energy without equal!

People who undergo aggressive therapies against cancers, chemotherapy or radiation, will benefit enormously by taking good daily doses of NAD+ to protect their cells and repair the damage more quickly.

Biotrends offers the most cutting edge and efficient products, thanks to the work of its professionals, but also because Biotrends was created by enthusiasts who want to rejuvenate themselves!

Biotrends is born to offer the means to rejuvenate to the greatest number, not to make money, and that makes all the difference, and guarantees us of the seriousness and the quality of their products 🙂

See: magazine “Health Science and Conscience” n° 10 of June 2017. This issue also contains a big file on diet. Hurry up to find it again in the kiosks.

Yours

Naturally Anti-Aging Cream

Skin Factor: an anti-aging cream that naturally repairs, rejuvenates and brings back the lost radiance

SkinFactor offers amazing anti-aging and skin rejuvenating benefits. Side effects? A glowing, nourished and youthful skin.

The SkinFactor cream by Biotrends comes with two primary ingredients – Hexapeptide 3 and Epidermal Growth Factors (EGFs); along with glycerol and rose water.

All these skin-healthy components come together and work to reduce stress lines, reclaim lost moisture and heal damaged skin.

Result? A naturally glowing, revitalized and healthy looking skin without using any harsh chemicals and without any side-effects.

Hex 3 and EGFs are peptides – long chain amino acids – that are making great waves as superior anti-aging ingredients. Peptides signal cells to produce more collagen, repair damaged skin and fight wrinkles and fine lines. They do all that you could ask for to give your skin a new lease of life and make your skin more beautiful.

Epidermal Growth Factors are The ultimate skin rejuvenators

Epidermal growth factor or EGF as it is commonly called is basically a polypeptide – a chain of amino acids. EGFs are naturally occurring proteins known to stimulate cellular growth, healing and regeneration [1].

Growth factors were discovered in 1986 by two scientists, Stanley Cohen and Rita Levi-Motalcini, who received a Nobel Prize in Medicine for this breakthrough discovery. Their work gave revolutionary insights into the ways in which cells communicate with each other during growth, differentiation, repair and healing.

EGFs encourage cellular repair and wound healing in the skin – meaning it possess incredible rejuvenating and age-reversing potential, all tapped and unlocked in our unique SkinFactor cream. When we say skin damage or repair, it includes injury or damage due to sun exposure, pollution, free radicals, burns, surgery and ageing. Basically, it works with unhealthy, damaged skin that needs repair and regeneration.

The skin repair and rejuvenating benefits offered by EGFs shouldn’t be surprising. These growth factors are naturally present in the fibroblasts of our skin, which are the cells responsible for producing extracellular matrix and collagen. A body of randomized controlled clinical trials suggest that topical growth factors achieve similar outcomes as endogenous growth factors, and reduce the signs associated with skin ageing. A recent 2016 article reports that topical growth factors are able to help “due to their capacity to promote dermal fibroblast proliferation and to stimulate extracellular matrix formation” [2].

How EGFs function?

Epidermal growth factors support the skin’s natural regeneration and revitalization process

It all starts with the inflammation stage. Inflammation may sound obnoxious but it is an integral mechanism of how our body responds to any injury, damage or infection. During this stage, our body transitions into an aggressive repair and healing mode by initiating certain events, and the release of EGFs is one of them. EGFs kick start cascading bio-chemical pathways that initiate tissue repair and regeneration in damaged skin cells – by signalling other cells and chemicals to arrive at the site of injury – and leads to:

  • Increased production of collagen and other elastin fibres
  • Growth of new blood vessels to supply nutrient and oxygen rich blood at the site of repair
  • Extracellular matrix deposition and remodelling

What does it mean for your skin? Creams with growth factors help in repairing damaged skin fast, boost collagen formation for increased elasticity, reduces age spots and pigmentation, and improve the skin’s integrity.

What is Hexapeptide -3?

Hexapeptide -3: Prevents the formation of expression lines and wrinkles

Like EGFs, Hexapeptide -3 is also a polypeptide. It is a chain of 6 amino acids and a fragment of SNAP-25, a substrate of Botox. Evidence suggest that hexapeptide-3 can reduce wrinkles by 30%, and within an impressive time frame of only 30 days.

How Hex 3 works: Relaxes facial muscles

We all age with time. Fine lines and wrinkles are a part of this natural ageing process. However, some external factors – such as over exposure to sun, smoking, drinking, stress and an unhealthy lifestyle – can play a major role in making our skin to age faster. There is another factor that contributes big time to this process – repetitive facial expressions.

Expressions like smiling, squinting and frowning make the underlying muscles contract, resulting in folds or creases. When we are young, our skin is more elastic and it has the flexibility to easily spring back into its place. But an ageing skin is no longer this versatile and flexible. Repetitive contractions of the facial muscles eventually pave way for permanent grooves and creases on the face. Well, we are all familiar with these furrows. Call them frown lines, crow’s feet or laugh lines, in the end they are something that we all dread. So how Hex 3 can help?

Hex 3 works by relaxing the facial muscles. Basically, Hex 3 blocks the communication that travels from nerves to muscles asking the muscles to contract. How?

At the neuromuscular junction, a site where a nerve fibre meets a muscle fibre:

  • Nerve cells release certain neurotransmitters (Acetylcholine and others)
  • The released chemicals transmit signals that activate a muscle fibre to contract.
  • These neurotransmitters are secreted with the help of SNARE proteins
  • SNARE proteins help nerves to release the neurotransmitters through a process called exocytosis.

Hexapeptide 3 interferes with the working of SNARE protein complex (it mimics one the proteins in the complex) and disrupts their function. Result? Chemicals that signal the muscle to contract are blocked, muscles don’t contract – thus preventing the formation of expression lines or wrinkles.

SkinFactor also contains glycerol and rose water

Glycerol

Glycerol is great for skincare not for one but many reasons:

  • Acts as a highly effective moisturizer
  • Soothes dry, damaged skin
  • Keeps skin nourished and hydrated
  • Enables young skin cells to mature and develop properly

Pure rose water

Besides imparting a delightful fragrance of fresh roses, pure rose water has a lot of skin benefits to offer:

  • Has anti-inflammatory and antioxidant properties
  • Soothes and cools irritated skin
  • Maintains moisture as well as pH balance in the skin
  • Stops bacterial growth, helps in reducing acne and pimples
  • Improves skin tone

Why should you buy SkinFactor?

Because it is one factor missing in your challenge to maintain a healthy, nourished and glowing skin without any side effects at all!!

What makes SkinFactor a unique antiaging cream? Synergistic combination of Hexapeptide, Epidermal growth factors, glycerol and rose water – a rare blend in any skincare product!

  1. Hexapeptide 3 relaxes facial muscles – to avoid expression wrinkles
  2. EGFs stimulate skin regeneration and repair – for better healing against sun exposure and other damage inflicted to skin
  3. EGFs also boosts collagen production – for a firm, elastic and supple skin
  4. Glycerol and rose water helps in keeping skin hydrated, nourished and overall healthy.
  5. Results as effective as Botox application. The big difference?  No toxic side effects, easy application and affordable

 

References:

  1. Barrientos S et al. Growth factors and cytokines in wound healing. Wound Repair Regen. 2008 Sep-Oct; 16(5):585-601. doi: 10.1111/j.1524-475X.2008.00410.x.
  2. Frank Dreher. Topical Growth Factors for Skin Rejuvenation. Textbook of Aging Skin. Pp 1-12. April 2016.

NAD+ promotes overall health and longevity

NAD+ is creating quite a buzz in scientific circles.

Be forever young, stay at the same age feel younger than you are, stop aging!

Nicotinamide Adenine Dinucleotide, or NAD+ for short, is used by every cell in the body. NAD+ is important because it is a big player in the energy production process within the mitochondria. Sirtuins, a class of enzymes well known for their role in regulating ageing, need NAD+ to do their job. And, research tells us that the tight integration between NAD+ availability and sirtuins activity is the major driver in controlling ageing factors and promoting longevity.

NAD+: A co-enzyme used by every living cell

NAD exists in two forms: NAD+ and NADH. NAD+ is the oxidized form of NAD. NAD+ levels decrease as we age, and scientists have established a solid inverse relationship between NAD+ levels and ageing.

How does NAD+ work?

NAD+ sits at the heart of the energy production process that occurs within the mitochondria – aptly called the ‘powerhouse of the cells’. How does it help in maintaining overall health and promoting longevity?

  • NAD+ improves mitochondrial functions
  • NAD+ activates Sirtuins, family of enzymes linked with regulating lifespan

Let’s explore the role of NAD+ in more detail.

NAD+ improves mitochondrial health

The cells in our body go through cycles of reproduction growth, repair and survival – and require tremendous energy to perform these myriad functions. Every cell in our body produces its own energy with all the action taking place in the mitochondria – tiny organelles within the cells. The energy is produced in a complex series of redox reactions, where NAD+ keeps shuttling between two forms; NAD+ and NADH.

NAD+ accepts electrons that are released during the burning or oxidation of organic fuels (fats, glucose), and in turn is reduced to NADH. NADH transfers the electrons in this ongoing chain of redox reactions – eventually resulting in ATP synthesis in a complicated yet highly organized process called oxidative phosphorylation. Clearly, NAD+ drives this process forward – that is, it facilitates the transfer of energy from the food to our cells – and thereby helps cells carry out their numerous functions that are so critical to our sustenance, physical and mental well-being, and survival.

 

What happens when NAD+ levels decline?

Reduced levels of NAD+ means the energy transfer takes a massive hit – resulting in impaired mitochondrial functions and increased oxidative stress, which in turn manifest as:

  • Reduced energy (ATP) production
  • Reduced heat production
  • Increased generation of damaging free radicals, causing damage to DNA, proteins and lipids
  • Reduced cellular ability to perform basic functions: healthy division, growth and repair damage triggered by oxidative stress
  • Disturbed metabolic pathways (causing metabolic imbalance, insulin resistance, unbalanced sugar levels)
  • Chronic inflammation
  • Cognitive dysfunction

Mitochondrial dysfunction is believed to be one of the main reasons behind the symptoms of ageing, such as physical and cognitive decline, loss of energy and fatigue. Not surprisingly, with the mitochondria not working as smoothly and efficiently, cells become vulnerable to oxidative stress, hence trailed by premature ageing and age associated diseases such as heart failure, type 2 diabetes, insulin resistance, obesity, hearing loss, vision loss, Alzheimer’s disease and cancer.

NAD+ and Sirtuins: NAD+ activates Sirtuins

Internal structure of a cell.

Playing important roles in the process of ageing and mitochondrial fitness are sirtuins – SIR2 or silent information regulator 2 proteins. This family, so far as current research has identified, consists of seven enzymes (SIRT1–7). In mammals, Sirtuins control how the body responds to energy metabolism and stress – two factors at the core of the ageing process.

While sirtuins are found in different compartments of a cell, such as nucleus, cytoplasm and mitochondria, and may have unique functions, the predominant role of Sirtuins can still be summed up as:

  • To protect cells from stress and,
  • Enhance cellular capacity to develop resistance against stressors, survive and self-preserve, thus contributing to increasing life span.

And Sirtuins are dependent on NAD+ for their activity. In fact, Sirtuins also act as a substrate for other enzymes including bacterial DNA ligase, Poly-ADP-ribose Polymerases (PARPs) and CD38, with all these enzymes playing important role in cell survival. This competitive use of NAD+ among so many related ‘consumer’ enzymes is also one of the factors why NAD+ levels decline as we age.

Sirtuins: The magic behind the effects of calorie restriction (CR)

A low-calorie diet is one of most effective and proven strategies to keep ageing at bay. Even long before modern scientific studies, many traditional systems of medicine, such as Ayurveda, have long believed that calorie restriction (CR) is the road to achieve a long, healthy life. So, what is the mystery behind CR and anti-ageing benefits? How come depriving the body of calories can make you live longer?

It seems that Sirtuins hold the key to this puzzle. Of course, one of the theories backing the CR effect is that it reduces the formation of free radicals, invariably formed when our cells use nutrients and oxygen to create energy during the oxidative phosphorylation process. It is something like when a vehicle burns fuel and releases smoke as a by-product. With reduced generation of free radicals, cellular structures experience less oxidative damage, and function better.

However, more and more studies indicate there is something more interesting happening at the backend. It appears that CR drives some very targeted bio-chemical responses, which helps cells preserve themselves and survive under unfavorable conditions. And no points for guessing, sirtuins are believed to drive these biological responses.

CR achieves the anti-ageing effect by activating sirtuins. To be precise, CR increases the expression of SIRT1, SIRT3 and SIRT5, enzymes that are known to regulate the expressions of genes associated with ageing. So, increasing the bio-availability of cellular NAD+ and increasing Sirtuins activity to extend the lifespan (that mimics CR mechanism) makes complete sense as a strategy to combat ageing and diseases of ageing.

NAD+ and Sirtuins: The Complex Relationship

Sirtuin 6 protein. linked to longevity.

In the mitochondria, NAD+ levels mirror the energy status of the cell. And sirtuins function as sensors; detecting the variations in the NAD+ levels and processing this information to help cells adapt and survive fluctuating energy requirements. NAD+ availability decreases as we age, negatively impacting Sirtuin activity.

The complex relationship between NAD+ and Sirtuins helps in controlling metabolic and longevity pathways. Studies show that this tight integration between the availability of NAD+ and Sirtuins activity helps to:

  • Maintain the communication between the nucleus and mitochondria at a cellular level and;
  • Maintain the inter-tissue communication, more specifically between the hypothalamus and adipose tissue at a systemic level.

The integration of NAD+ and sirtuins orchestrates this important communication, both at cellular as well as systemic level. If this orchestration works smoothly, the result is melodious harmony that keeps cells healthy. However, with low NAD+ levels, this communication gets disrupted. Cells lose their ability to create sufficient energy and the process of ageing accelerates. Dr. David Sinclair, Harvard Medical School Professor of Genetics commented that “this communication network is like a married couple—when they are young, they communicate well, but over time, living in close quarters for many years, communication breaks down. And just as with a married couple, restoring communication solved the problem.”

Overall, NAD+ dependent Sirtuins protects against premature ageing and associated diseases through various mechanisms, such as:

  1. Deacetylation of proteins like histones
  2. Regulating metabolism and circadian rhythm
  3. DNA repair
  4. Boosting mitochondrial functions and response in the face of external or internal stress, for example fasting and oxidative damage
  5. Reducing inflammation in the body

NAD+ Benefits:

Boosts energy and fights premature ageing

Improves mitochondrial efficiency. Enhances energy metabolism. Reduces Oxidative stress.

Emerging research and scientific studies are all pointing to the role of NAD+ in improving human lifespan, quality of life and all-inclusive health. The evidence is promising and shows that boosting NAD+ levels can help us achieve a healthy and disease free life. Since oxidative stress and mitochondrial dysfunction are implicated in ageing and most heath conditions, NAD+ indeed is fast emerging as a real possibility to prevent and even reverse a wide range of chronic ailments, including:

  • Metabolic disorders, such as obesity, insulin resistance, type 2 diabetes
  • Neuro-degenerative disorders, such as Alzheimer’s disease, Parkinson’s disease, dementia
  • Cancer
  • Cardiovascular disorders
  • Inherited mitochondrial diseases
  • Vision loss
  • Hearing loss

 

References

    1. Imai et al. NAD+ and Sirtuins in Ageing and Disease. Trends in Cell Biology. 2014.
    2. Akiko Satoh, Liana Stein, Shin Imai. The Role of Mammalian Sirtuins in the Regulation of Metabolism, Ageing, and Longevity. Handb Exp Pharmacol. 2011; 206: 125–162.
    3. Gomes et al. Declining NAD+ Induces a Pseudohypoxic State Disrupting Nuclear-Mitochondrial Communication during Ageing. Cell. 2013.
    4. Shin-ichiro Imai, Leonard Guarente. It takes two to tango: NAD+ and sirtuins in ageing/longevity control. Nature. Ageing and Mechanisms of Disease. August 2016

Resveratrol as in Nouvelle-Vie

Resveratrol –a wonder compound

Getting to know the compound ‘Resveratrol’ is quite interesting, as it holds the key to the secret of ‘graceful aging’. It has been identified as the root cause of the  ‘French paradox’. If you have never heard of the French Paradox, it is the observation that French people consume creamy pastries, sauces and high saturated fats and still manage to have a healthier heart than people from other cultures – that is, they have a low incidence of heart diseases. Research shows that consumption of red wine is the probable explanation. Red wine is produced using the skin of red grapes which is rich with Resveratrol, a powerful antioxidant required for restoring and preserving good health. (Wein 2013)

 

What is Resveratrol?

It is a polyphenol produced from plants. Polyphenols are a group of micronutrient compounds which protect against diseases. Resveratrol is produced from plants in response to any injury, stress, fungal infection or UV exposure  (Higdon 2015). It is an antioxidant also known as ‘phytoalexin’. Resveratrol, when consumed, can provide a very effective protection to humans as well.

How Resveratrol works?

In the past decade, Resveratrol has been added to the list of effective nutritional supplements. Scientists have continued researching how Resveratrol works in the body and keeps the body younger and healthier. In short it is an antioxidant, it does what an antioxidant does and protects the body against the harmful effects of free radicals. These free radical are responsible for all the damage  to the body that one can think of, with one major fallout being premature aging.

When the body undergoes any kind of stress, it triggers ‘oxidative stress’ with the generation of these ‘free radicals’ leading to a negative impact on the body (Skerrett 2012)

Having mentioned that, let’s clarify that not all antioxidants are the same. What makes Resveratrol the ‘best dressed’ antioxidant is the mechanism by which it protects the body. The added attraction is that it also promotes the normal process of cell division and relieves inflammation which causes signs of premature aging.

 

The link between mitochondria, aging and Resveratrol

‘Mitochondria’ is an organelle in the cell, also known as the ‘powerhouse’ of the cell. It even contains some DNA and produces ATP (adenosine triphosphate) as a result of the respiratory chain. ATP are the energy shots for the body, and all the functions in the body require energy produced by this ATP. Mitochondrial DNA is affected largely by free radicals. Mitochondrial DNA mutations are responsible for many diseases and most profoundly the appearance of the signs of aging. Damage to mitochondria results in sudden cell death, but by slowing down aging of mitochondria through calorie restriction and exercise, all body aging can be slowed down (Chistiakov 2014).

So, what is all this fuss about mitochondrial DNA? The link here is that Resveratrol has been found to protect the body from the damaging effects of a high fat diet. High caloric food ultimately damages mitochondria and we end up with premature aging, and Resveratrol can neutralize all these effects and restore youthfulness of the body . (Mattison 2014) Read that again – Resveratrol CAN neutralize the effects  of aging and restore youth!

 

Resveratrol and premature aging

Premature aging occurs mainly due to one or more of these 4 factors: a weak immune system, poor digestive system, inflammation and/or oxidative stress. Resveratrol acts on at least 3 of these factors to reduce the signs of premature aging – the immune system, inflammation and oxidative stress.

Other remarkable health benefits of Resveratrol

More than 4,400 studies have been published discussing the positive effects of Resveratrol on our health, with  anti-aging benefits just the  tip of the iceberg. Adding to this, Resveratrol is known to:

  • activate a survival gene (Sirtuin 1) in the brain
  • facilitate the connections between the brain cells, and
  • suppresses age-related memory decline (Markus 2008)

Here is short list of why you should be taking a Resveratrol supplement, and what it does:

  • a powerful heart health protectant, known to protect against cardiac diseases and promote the restoration of blood flow in hypertensive patients.
  • protects the inner lining of arteries (endothelium) by oxidative damage and also keeps the normal production of nitric oxide in the body. Nitric oxide is responsible for the dilation of arteries which maintains the blood flow.
  • protects the body against inflammatory agents and boosts the immune system.
  • found effective in maintaining dental health as well as mental health, and helps  maintain muscle health.
  • suppresses cancer by interfering with the division of cancer cells and increasing cancerous cell death.
  • protects against obesity, diabetes, lowers bad cholesterol (LDL) levels and can reduce chances of blood clotting.
  • can increase the production of a serum (Sirtuin 1), the serum that speeds up the energy producing mitochondria and ultimately blocks certain diseases
  • found effective against treatment of Alzheimer’s disease.

 

Sources of Resveratrol

Resveratrol is present in  some fruits and vegetables, and of course it is in the skin of red grapes, but the most effective and absolute way to ensure your body has the Resveratrol it needs to combat aging and the associated breakdown of the immune system is to take a quality supplement each day.

 

Dosage and how to get maximum from your resveratrol?

The recommended daily intake of Resveratrol is  250 mg. If red wine is considered the source, about 170 standard glasses of red wine contain ‘almost’ the recommended amount. It is obviously far healthier and more economical – although perhaps less interesting – to take a daily supplement from a reliable source.

It is not only a potential ‘anti-aging’ agent which will reduce fine lines, wrinkles and make you ‘look’ younger, you WILL feel younger. It protects  your entire body, including your brain, from the  ill effects of aging by repairing and buffering mitochondrial damage.

References

    1. Wein H. (2013) How Resveratrol May Fight Aging National Institutes of Health
    2. Higdon J. (2005) and reviewed by Espin HC (2015) Resveratrol Linus Pauling Institute, Oregon State University
    3. Skerrett PJ, (2012), Resveratrol-the hype continues. Harvard Medical School Harvard Health Publications
    4. FREE PDF: Chistiakov DA, Obenin IA, Evin VV, Ekhov AN,Obryshev YV (2014) Mitochondrial Aging and Age-Related Dysfunction of Mitochondria BioMed Research International Volume 2014, Article ID 238463
    5. Mattison A. (2014) Resveratrol prevents high fat/sucrose diet-induced central arterial wall inflammation US National Library of Medicine 2014 Jul 1; 20(1): 183-9
    6. Markus A, Morris BJ (2008) Resveratrol in prevention and treatment of common clinical conditions of aging US National Library of Medicine 2008 Jun; 3(2): 331–339.
    7. Castillo M. (2013) Resveratrol does provide anti-aging benefits CBS News